his-selecttm nickel affinity gel (Millipore)
90
Structured Review
Millipore
his-selecttm nickel affinity gel
His Selecttm Nickel Affinity Gel, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/his-selecttm+nickel+affinity+gel/his+select+nickel+affinity+gel/pm28980353-44-9-13
Average 90 stars, based on 1 article reviews
His Selecttm Nickel Affinity Gel, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/his-selecttm+nickel+affinity+gel/his+select+nickel+affinity+gel/pm28980353-44-9-13
Average 90 stars, based on 1 article reviews
his-selecttm nickel affinity gel - by Bioz Stars,
2026-10
90/100 stars
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other:Article Title: Characterization of a new family of protein kinases from Arabidopsis containing phosphoinositide 3/4-kinase and ubiquitin-like domains Article Snippet: At least two of the genes predicted to encode type II PI4K (phosphoinositide 4-kinase) in Arabidopsis thaliana (thale cress), namely AtPI4Kγ 4 and AtPI4Kγ 7, encode enzymes with catalytic properties similar to those of members of the PIKK (phosphoinositide kinase-related kinase) family.. AtPI4Kγ 4 and AtPI4Kγ 7 undergo autophosphorylation and phosphorylate serine/threonine residues of protein substrates, but have no detectable lipid kinase activity.. AtPI4Kγ 4 and AtPI4Kγ 7 are members of a subset of five putative AtPI4Ks that contain N-terminal UBL (ubiquitin-like) domains. Article Title: Structural Basis for the Golgi Association by the Pleckstrin Homology Domain of the Ceramide Trafficking Protein (CERT) Article Snippet: TheHis6-tagged CERTPHdomain was purified from the supernatant of the centrifuged lysate by metal-chelating chromatography, using Article Title: Δ1-Pyrroline-5-carboxylate reductase from Arabidopsis thaliana: stimulation or inhibition by chloride ions and feedback regulation by proline depend on whether NADPH or NADH acts as co-substrate. Article Snippet: The His-tagged protein was purified from clarified extracts by affinity chromatography with a His-SelectTM Nickel Affinity Gel ( Article Title: Substrate specificities of family 1 UGTs gained by domain swapping. Article Snippet: Family 1 glycosyltransferases are a group of enzymes known to embrace a large range of different substrates.. This study devises a method to enhance the range of substrates even further by combining domains from different glycosyltransferases to gain improved substrate specificity and catalytic efficiency.. Chimeric glycosyltransferases were made by combining domains from seven different family 1 glycosyltransferases, UGT71C1, UGT71C2, UGT71E1, UGT85C1, UGT85B1, UGT88B1 and UGT94B1. Article Title: Structure-Function Analysis of Mouse Purβ II Article Snippet: After enrichment on Article Title: Longitudinal analysis of allergen-specific IgE and IgG subclasses as potential predictors of insect bite hypersensitivity following first exposure to Culicoides in Icelandic horses. Article Snippet: *Department of Clinical Research and Veterinary Public Health, Vetsuisse Faculty, University of Berne, L€anggass-Strasse 124, Berne 3012, Switzerland †Department of Zoonoses, Faculty of Veterinary Medicine, Cairo University, PO Box 12211, Giza, Egypt ‡Institute for Experimental Pathology, Biomedical Center, University of Iceland, Keldur, Keldnavegur 3, Reykjavik 112, Iceland §Swiss Institute of Allergy and Asthma Research (SIAF), Obere Strasse 22, Davos 7270, Switzerland ¶Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca NY 14853, USA Correspondence: Eliane Marti, Department of Clinical Research and Veterinary Public Health, Vetsuisse Faculty, University of Berne, L€anggassStrasse 124, 3012 Berne, Switzerland.. E-mail: eliane.marti@vetsuisse.unibe.ch Article Title: Overproduction of an Arabidopsis aldo–keto reductase increases barley tolerance to oxidative and cadmium stress by an in vivo reactive aldehyde detoxification Article Snippet: In this work, we expressed an Arabidopsis thaliana-coded protein (AKR4C9) in transgenic barley to study its enzymatic activity and to enhance the reactive aldehyde neutralizing capacity (part of the oxidative stress tolerance) of transgenic plants.. Total leaf protein was extracted from transgenic plants expressing either C or N-terminally His-tagged aldo–keto reductase (AKR) enzyme and purified by affinity chromatography.. The Arabidopsis-coded enzyme showed moderate activity against the synthetic reactive aldehyde, glutaraldehyde, and low but detectable enzyme activity against fructose with a low Michaelis–Menten constant (Km value). Incubation:Article Title: p97/valosin-containing protein (VCP) is highly modulated by phosphorylation and acetylation. Article Snippet: Cells were lysed in a phosphate buffer (50 mm sodium phosphate (pH 7.8), 300 mm NaCl, 1% Nonidet P-40, 1 mm ATP, 10 mm β-mercaptoethanol, 100 μg/mL 4-(2-aminoethyl)-benzenesulfonyl fluoride (AEBSF), 20 mm benzamidine and a protease inhibitor cocktails (AEBSF, aprotinin, E-64, leupeptin, pepstatin and pepstatinA) (Nacalai Tesque). .. The lysates were incubated with HIS-SelectTM. |